(ii) Concentration of Substrate: (Cone of Soc Rate of E Action up to a limit (E is constant)


(a) Low Concentration:

• At low concentration of substrate the reaction rate is directly proportional to the substrate available. (b) High Concentration: If the enzyme concentration is kept constant and the amount of substrate is increased, a point is reached when any increase in the substrate does not increase the rate of reaction more, because at high substrate level all the active sites of the enzyme are occupied with substrate. Further increase in the substrate does not increase the reaction rate,

Maximum rate for enzyme at concentrations A and B

Active altes still available

Active sites almost all occupied. Enzyme is saturated Saturation of Active side V max

Rate of reaction

Active site not all occupied

Substrate Concentration

Effect of substrate concentration in the rate of Enzyme-control reaction

Imp. S/Q

Do you know why rate of enzyme action increases at high temperature and decreases at very high


Substrate concentration

(iii) Temperature: (Temperature o Rate of E Action upto a limit then rate decreases)

(a) Optimum Temperature:

All enzymes can work at their maximum rate at a specific temperature (7) called as optimum

temperature. For enzyme of human body 37°C is the optimum temperature.

(b) Low Temperature:

At very low temperature (0°C), the activity of the enzyme is minimum.

(c) High Temperature:

Beyond 45°C, usually the activity of enzyme is permanently stopped due to the denaturation (

A) of enzyme. and therefore, chemical reactions are ( ری ایکشن کو شروع کرنے والی توانائی کی کم سے کم مقدار) Heat provides activation energy •

accelerated () at high temperature. So rate of enzyme action increases.

(d) Heat as Kinetic Energy: Heat also supplies kinetic energy to the reacting molecules, causing them to move rapidly, thus the reactants move more quickly (76) and chances of their collision G) with each other are increased.

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